A Drosophila Neuromuscular Synaptogenesis Research Laboratory  
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Research ::  

A major goal in neuroscience is the understanding of organizational principles and the underlying communication and integration mechanisms leading to a functional nervous system. Many developmental processes involve massive cellular migrations, communication and reorganization. Today, it is accepted that extensive networking of cells and their molecular components result in formation of the brain. In the past, genetic and biochemical studies have identified molecules that facilitate accurate synaptic target recognitions as well as those responsible for pre- and postsynaptic specializations. However, the significance of explicit cellular motilities and required molecular integration within emerging cellular network needs to be further addressed. The laboratory’s main interest is the study of the dynamic nature of those cellular and molecularevents and how they are integrated to give rise to the synapse. Please read on and contact us if you have any comments, questions or suggestions.


  • Cellular Events
  • Molecular Events
  • Cell and Molecular Integration
  • Contact Us
Myopodia

The precision in synaptic matchmaking at the Drosophila neuromuscular system is ultimate, it displays a one-to-one single cell resolution. How the neurons and muscles accomplish this astounding precision is still a major challenge to neurobiology. However, our observations may shed some light into this process in vivo. Using three- and four-dimensional analysis in undissected, live Drosophila embryos expressing GFP-based bioprobes, we have observed highly animated actin-based micro processes (myopodia) that extend from muscle cells before neuromuscular synaptogenesis. This part of my research program is designed to understand the underlying cellular behavioral pattern of myopodia... <Learn More>
Myopodia Cluster

We have shown that every neuromuscular synapse in live embryos is preceded by a transient stabilization / clustering of presynaptic and postsynaptic filopodia from matched partners upon their initial contact. We call this new sub cellular space myopodial cluster. Our previous work reveals the extensive interdigitation at the myopodial cluster. However, in several mutant backgrounds (such as Prospero) that has severe motor neuron axon outgrowth from CNS myopodial clustering fails to occur. Furthermore, filopodial interactions between non-partner cells, though extensive and essential for proper axon guidance, do not lead to myopodial clustering. This part of my research program is aimed at the understanding of the underlying cellular events that leads to myopodial cluster formation and the role this new sub-cellular compartment plays prior and during synaptogenesis. <Learn More>

 

PSD95/Dlg >>

A strong molecular candidate for playing a role in the early stages of synaptogenesis is the Discs Large (DLG) protein. DLG has been shown to co-localize to the neuromuscular junction and is thought to be involved in organizing the postsynaptic density (Budnik et. al.). This task is facilitated by the fact that DLG posses many modular domains known to interact with other components involved in the synaptogenesis process (Fas II, Shaker, CamK-II). <Learn More>
Other Molecules >>

Additional information coming soon...

 

Pre-Synaptogenic Events:: Axon Targeting and Pathfinding >>

A precise synaptic matchmaking occurs between the 30 muscle cells and 34 motor neurons that are present in each hemisegment (half-segment) in the Drosophila embryo. Muscle enervation by motor neurons is a dynamic process determined known molecular interactions occurring at the neuron’s growth cone. Myopodia formation in the muscle is the site where the dynamic process of synaptogenesis takes place. This part of my research program is aimed at gaining a better understanding of innervation and its timing in a developmental context. This knowledge will allow us to gain in-depth understanding of the sequence of events as they unfold leading to the formation of a fully functional synapse. <Learn More> about the Drosophila Neuromuscular System:: <Learn More Myopodia> :: <Learn More Myopodial Clustering>
Synapse Fomation :: Synaptogenesis >>

After completion of the myopodia clustering behavior, stabilized myopodia dynamically interact with the corresponding innervating motoneuron axon to form a synaptic connection. <Learn More>
Post-Synaptogenic Events :: Synaptic Maturation and Plasticity >>

Even though this area of research is beyond the current scope of my research program, I will post more information soon...

 

Office

E-mail :: fcarrero uprm edu

Office Hours :: Wednesdays 8:00am - 9:00am
Office Location :: Biology Building B-204
Office Phone :: 787-832-4040 x3923

Lab

Laboratory Location :: Biology Building B-282B
Laboratory Phone :: 787-832-4040 x3940

Microscopy Center

Location :: Biology Building B-000
Phone :: 787-832-4040 x0000
Website :: http://